LSBio
N-Acylmannosamine-1-Dehydrogenase Antibody (Lyophilized, HRP)

Goat anti N-acylmanosamino-1-Dehydrogenase antibody was used to detect purified N-acylmanosamino-1-Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.

N-Acylmannosamine-1-Dehydrogenase Antibody (Lyophilized, HRP)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Reactivity:Bacteria
Format:Lyophilized
See all Pseudomonas N-Acylmannosamine-1-Dehydrogenase antibodies →
$564.00each
Catalog Number: LS-C744727-100

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Overview

Description
N-Acylmannosamine-1-Dehydrogenase antibody LS-C744727 is an HRP-conjugated goat polyclonal antibody to N-Acylmannosamine-1-Dehydrogenase from pseudomonas. It is reactive with bacteria and pseudomonas. Validated for ELISA, IP and WB.
Application
ELISA, IP, WB
Reactivity
Bac
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:5000 - 1:20000
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:500 - 1:5000

Target

Target
Pseudomonas N-Acylmannosamine-1-Dehydrogenase
Antigen Species
Bac
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, and purified and partially purified N-Acylmannosamine-1-Dehydrogenase derived from Pseudomonas.

Antibody

Host Species
Gt
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]
Immunogen Type
Recom

Format

Conjugate/Label
HRP
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
IEX

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.