Western blot analysis of extracts from LOVO cells, treated with H2O2 (100uM, 30mins), using Myosin regulatory light chain 2 (Phospho-Ser18) antibody.
MYL12A / MRCL3 Antibody (phospho-Ser18)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C826642-100
Catalog Number: LS-C826642
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- Description
- MRCL3 antibody LS-C826642 is an unconjugated rabbit polyclonal antibody to MRCL3 (MYL12A) (pSer18) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- MRCL3 | MLCB | MRLC3 | MYL2B | MLC-2B
- Recommended Dilution: ELISA
- 1:20000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P19105
- NCBI GenBank
- NM_006471 | NP_006462.1
- SwissProt
- P19105
Target
- Target
- Human MYL12A / MRCL3
- Antigen Species
- Human
- Epitope
- Myosin regulatory light chain 2 (Phospho-Ser18) antibody detects endogenous levels of Myosin regulatory light chain 2 only when phosphorylated at serine 18. (Human: Ser18; Mouse: Ser20; Rat: Ser19)
- Gene Name
- MYL12A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- The antiserum was produced against synthesized phosphopeptide derived from human Myosin regulatory light chain 2 around the phosphorylation site of serine 18 (A-T-SP-N-V).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl