Anti-Myc epitope Tag Antibody - Western Blot. Anti-Myc epitope tag polyclonal antibody detects both AMINO and CARBOXY terminal linked Myc-tagged recombinant proteins by western blot. Polyclonal rabbit host anti-Myc epitope tag antibody was diluted to 1.0 ug/ml to detect either recombinant protein. 4-20% gradient gels were used to resolve the proteins by SDS-PAGE. The proteins were transferred to nitrocellulose using standard methods. After blocking, the membranes were probed with the primary antibody overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 45 min at room temperature (Green, 800 nm channel). Pre-stained molecular weight markers are also shown (lane M, Red, 700 nm channel). LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.