Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48.8 kDa c-Myc protein using Rabbit Anti-c-Myc Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Myc Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.8 kDa. Other Band(s): due to post translational modifications.
MYC / c-Myc Antibody (FITC)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$482.00each
Catalog Number: LS-C776511-100
Catalog Number: LS-C776511
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- Description
- C-Myc antibody LS-C776511 is an FITC-conjugated rabbit polyclonal antibody to human c-Myc (MYC). Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BHLHe39 | C-Myc | MRTL | MYC | Myc proto-oncogene protein | Proto-oncogene c-Myc | Rats1 | Transcription factor p64 | v-myc
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P01106
- NCBI GenBank
- NM_002467 | NP_002458.2
- SwissProt
- P01106
Target
- Target
- Human MYC / c-Myc
- Antigen Species
- Human
- Epitope
- Detects 48 kDa, additional bands due to post translational modifications.
- Gene Name
- MYC
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide of Human c-Myc (220-320 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Fluorescein Isothiocyanate
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS