Western blot of Rabbit c-Myb pT486 antibody. Lane 1: Cos7 cells were either null for c-Myb. Lane 2: Cos7 cell lysates transfected with c-myb, no treatment. Lane 3: Cos7 cell lysates transfected with c-Myb and treated with okadaic acid to include phosphorylation of c-Myb. Load: 35 ug per lane. Primary antibody: The left blot was probed with anti-c-Myb antibody, the right blot was probed with anti-c-Myb pT486 antibody at 1:2500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: PBS-Tween (0.05%) overnight at 4C. Predicted/Observed size: 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrow): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.
MYB / c-Myb Antibody (phospho-Thr486)
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
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- Description
- C-Myb antibody LS-C153853 is an unconjugated rabbit polyclonal antibody to mouse c-Myb (MYB) (pThr486). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Ms
- Predicted Reactivity
- Horse, Human, Mammal (Other), Non-Human Primate, Pig, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C-myb_CDS | C-myb8B_CDS | C-myb protein (140 AA) | C-myb10A_CDS | C-myb13A_CDS | Efg | MYB | Transcriptional activator Myb | C-myb | C-myb14A_CDS | Cmyb | Proto-oncogene c-Myb
- Recommended Dilution: ELISA
- 1:5000 - 1:16000
- Recommended Dilution: WB
- 1:2500
- UniProt Number
- P10242
- NCBI GenBank
- NM_005375 | NP_005366.2
- SwissProt
- P10242
Target
- Target
- Mouse MYB / c-Myb
- Antigen Species
- Mouse
- Epitope
- Reactivity occurs against mouse c-Myb pT486 protein and the antibody is specific for the phosphorylated form of the protein. This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. Reactivity with non-phosphorylated mouse c-Myb is minimal by ELISA and western blot. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence. Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence. Cross-reactivity with c-Myb from other sources has not been determined.
- Gene Name
- MYB
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.91 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl