Anti-Mesothelin Antibody - Immunohistochemistry. Immunohistochemistry using anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission fromClin. Cancer Res. 11(16):5840-6.
MSLN / Mesothelin Antibody (clone MB-G10)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C154007-100
Catalog Number: LS-C154007
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- Description
- Mesothelin antibody LS-C154007 is an unconjugated mouse monoclonal antibody to human Mesothelin (MSLN). Validated for ELISA, IHC and WB. Cited in 1 publication.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CAK1 antigen | MPF | SMRP | Mesothelin | MSLN | CAK1
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: IHC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q13421
- NCBI GenBank
- NM_013404 | NP_037536.2
- SwissProt
- Q13421
Target
- Target
- Human MSLN / Mesothelin
- Antigen Species
- Human
- Gene Name
- MSLN
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- MB-G10
- Isotype
- IgG2a
Immunogen
- Immunogen
- This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA. Onda M, Willingham M, Nagata S, Bera TK, Beers R, Ho M, Hassan R, Kreitman RJ, Pastan I. Clinical cancer research : an official journal of the American Association for Cancer Research. 2005;11:5840-6. PubMed: 16115924