LSBio
Mouse Anti-Rabbit IgG Secondary Antibody (HRP)

Western blot of Peroxidase conjugated Mouse Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase mouse secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.

Mouse Anti-Rabbit IgG Secondary Antibody (HRP)

Secondary AntibodyLSBio Guarantee
Antibody:Mouse Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Rabbit
Format:Lyophilized
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$421.00each
Catalog Number: LS-C60914-1

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Overview

Description
Rabbit IgG antibody LS-C60914 is an HRP-conjugated mouse polyclonal antibody. Validated for ELISA, IHC and WB. Cited in 1 publication.
Application
DB, ELISA, IHC, WB
Reactivity
Rb
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: WB
1:1000 - 1:10000

Target

Target
Rabbit IgG
Antigen Species
Rabbit
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Mouse Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against or Goat, Human and Mouse Serum Proteins.

Antibody

Host Species
Mouse
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Rabbit IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl
Bmi-1 in gallbladder carcinoma: Clinicopathology and mechanism of regulation of human gallbladder carcinoma proliferation. Jiao K, Jiang W, Zhao C, Su D, Zhang H. Oncology letters. 2019 August;18:1365-1371. PubMed: 31423199 PMC: PMC6607302

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