LSBio
Mouse Anti-Rabbit IgG Secondary Antibody (AP)

Dot Blot of Mouse anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed. Antigen: Rabbit IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Mouse anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed at 1:1,000 for 1 HR at RT.

Mouse Anti-Rabbit IgG Secondary Antibody (AP)

Secondary AntibodyLSBio Guarantee
Antibody:Mouse Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Rabbit
Format:Liquid
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$448.00each
Catalog Number: LS-C60915-1

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Overview

Description
Rabbit IgG antibody LS-C60915 is an AP-conjugated mouse polyclonal antibody. Validated for DB, ELISA, IHC and WB.
Application
DB, ELISA, IHC, WB
Reactivity
Rb
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:500 - 1:2500

Target

Target
Rabbit IgG
Target Heavy Chain
IgG
Antigen Species
Rb
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Mouse Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human and Mouse Serum Proteins.

Antibody

Host Species
Ms
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Rabbit IgG whole molecule
Immunogen Type
Native

Format

Conjugate/Label
AP
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze.
Recommended Storage Buffer
0.05 M Tris-Chloride|0.15 M NaCl

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