Anti-Human IgA Secondary Antibody
Catalog Number: LS-C758146-1
1 mg / $306.00
Antibody: Human Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western Blot of Mouse anti-Human IgM Fc5 mu antibody. Lane 1: Human IgM. Lane 2: Human IgG. Lane 3: Human Fc. Lane 4: Human Fab. Lane 5: Human Fab2. Lane 6: Human IgM Fc5 mu . Load: 50 ng per lane. Primary antibody: Human IgM Fc5 mu monoclonal antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicated/Observed size: 78 kDA, 78 kDa for Human IgM.
Mouse Anti-Human IgM Secondary Antibody (clone 22G1.H8)
Secondary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$384.00each
Catalog Number: LS-C209273-100
Catalog Number: LS-C209273
For bulk orders or custom formulations:
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- Description
- Human IgM antibody LS-C209273 is an unconjugated mouse monoclonal antibody. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: ELISA
- 1:1000 - 1:10000
- Recommended Dilution: WB
- 1:1000 - 1:2000
Target
- Target
- Human IgM
- Antigen Species
- Human
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5µ. No reaction was observed against Human IgG, Human IgA, Human Fc, Human Fab or Human Fab2.
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 22G1.H8
- Isotype
- IgG2b
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Anti-Human IgM Fc5µ was produced by repeated immunization with Human IgM Fc5µ fragment in mice.
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl