LSBio
Monoclonal Rabbit anti-Human CASP3 / Caspase 3 Antibody (N-Terminus, IHC, IF, WB)

Monoclonal Rabbit anti-Human CASP3 / Caspase 3 Antibody (N-Terminus, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Monoclonal
Applications:Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human CASP3 / Caspase 3 antibodies →
$724.00each
Catalog Number: LS-C12477-100

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Overview

Description
Caspase 3 antibody LS-C12477 is an unconjugated rabbit monoclonal antibody to human Caspase 3 (CASP3) (N-Terminus). Validated for Flow, IF, IHC, IP and WB.
Application
FC, IF, IHC, IHC-F, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Apopain | CASP-3 | CASP3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama
Recommended Dilution: FC
1:200
Recommended Dilution: IP
1:50
Recommended Dilution: WB
1:1000
UniProt Number
P42574
SwissProt
P42574

Target

Target
Human CASP3 / Caspase 3
Antigen Species
Human
Epitope
Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases.
Gene Name
CASP3

Antibody

Host Species
Rabbit
Clonality
Monoclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide (KLH coupled) corresponding to N-Terminus residues adjacent to (Asp175) in human caspase-3.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Volume
100 Microliter
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
10 mM HEPES|150 mM NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.