LSBio
Monoclonal Mouse B cell Antibody (clone Ki-B1R, IHC)

Monoclonal Mouse B cell Antibody (clone Ki-B1R, IHC)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin
Reactivity:Rat
Format:Lyophilized
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$718.00each
Catalog Number: LS-C96406-50

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Overview

Description
B cell antibody LS-C96406 is an unconjugated mouse monoclonal antibody to rat B cell. Validated for IHC.
Application
IHC-F, IHC-P
Reactivity
Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: IHC-F
1:400
Recommended Dilution: IHC-P
1:10

Target

Target
B cell
Epitope
Ki-B1R recognizes a surface antigen of 47kDa molecular weight, which appears as a single band under reducing conditions in SDS-PAGE. The epitope has not been further characterized. Antigen distribution: Isolated cells: In a test comparing the immunophenotype of rat blood lymphocytes, Ki-B1R recognised 19% + 3% of peripheral blood lymphocytes (n=5). In the same test, 18% +/- 3% of the lymphocytes stained positive for sIg, whereas 79% +/- 3% stained positive with Ki-T1R, the rat pan T-cell marker. Tissue sections: Ki-B1R shows a staining pattern similar to that of anti-rat immunoglobulin markers. Follicular mantle B- lymphocytes are strongly positive whereas staining of germinal centre B-lymphocytes depends on the immunological status of the lyphoid organ. In the gastrointestinal tract, the typical mucosa-associated lymphoid tissue (MALT) selectively displays the B-follicles, mostly those containing prominent germinal centres. The reactivity pattern of Ki-B1R does not change when applied in vivo. Specificity: Rat: mature B-lymphocytes, plasma cells. Other species: not tested.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
Ki-B1R

Immunogen

Immunogen
Lymphocytes
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from PBS, pH 7.2, 5 mg/ml BSA, 0.09% Sodium Azide
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.