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Monoclonal Mouse anti-Rat PECAM-1 / CD31 Antibody (clone TLD-3A12, IHC, IF, WB)

IHC of rat spleen cryosection with Mouse anti-Rat CD31 (MOUSE ANTI RAT CD31). This image was taken for the unmodified form of this product. Other forms have not been tested.

Monoclonal Mouse anti-Rat PECAM-1 / CD31 Antibody (clone TLD-3A12, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Western Blot
Reactivity:Rat, Rhesus Macaque
Format:Liquid
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$415.00each
Catalog Number: LS-C43483-0.25

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Overview

Description
CD31 antibody LS-C43483 is an unconjugated mouse monoclonal antibody to CD31 (PECAM-1) from rat. It is reactive with rat and rhesus monkey. Validated for ELISA, Flow, IF, IHC and WB. Cited in 1 publication.
Application
ELISA, FC, IF, IHC, IHC-F, WB
Reactivity
Rhes, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CD31 | CD31 antigen | EndoCAM | PECA1 | PECAM1 | PECAM-1 | GPIIA
Recommended Dilution: FC
1:10 - 1:100
Recommended Dilution: IHC-F
1:10 - 1:100
UniProt Number
P16284
SwissProt
P16284

Target

Target
Rat PECAM-1 / CD31
Antigen Species
Rt
Epitope
Recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes. Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. This clone has been reported as suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2 M boric acid, pH7.0. Has been shown to be cross-reactive with endothelial cells derived from rhesus macaque.
Gene Name
PECAM1

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
TLD-3A12
Isotype
IgG1

Immunogen

Immunogen
Activated, Lewis rat derived microglial cells
Immunogen Type
Cell

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Prot G

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS
The effect of a silk Fibroin/Polyurethane blend patch on rat Vessels. Kazumi Shimada, Akira Higuchi, Ryota Kubo, Tomoaki Murakami, Yasumoto Nakazawa, Ryou Tanaka. Organogenesis. 2017 Oct;13:115-124. PubMed: 28933641 PMC: PMC5669211

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