LSBio
Monoclonal Mouse anti-Human WNT1 Antibody (clone 13F9, Internal, WB)

Anti-Wnt1 Monoclonal Antibody - Western Blot. Western blot of Protein A purified anti-Wnt1 monoclonal antibody shows detection of Wnt1 protein in mouse testis lysate. The results show specific binding corresponding to the ~41 kD Wnt1 protein. Primary antibody was used at a 1:500 dilution. Personal communication, Stephen Brown, Brown University.

Monoclonal Mouse anti-Human WNT1 Antibody (clone 13F9, Internal, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
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$586.00each
Catalog Number: LS-C154005-100

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Overview

Description
WNT1 antibody LS-C154005 is an unconjugated mouse monoclonal antibody to human WNT1 (Internal). Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu
Predicted Reactivity
Bovine, Chicken, Dog, Mammal (Other), Mouse, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
INT1 | Proto-oncogene Int-1 homolog | Proto-oncogene Wnt-1 | WNT1
Recommended Dilution: ELISA
1:4000 - 1:20000
Recommended Dilution: WB
1:175 - 1:250
UniProt Number
P04628
SwissProt
P04628

Target

Target
Human WNT1
Antigen Species
Human
Epitope
This antibody reacts with human and mouse Wnt1 protein. A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, opossum and rat based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology. Cross-reactivity with Wnt1 from other sources has not been determined.
Gene Name
WNT1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
13F9
Isotype
IgG1
Light Chain
Kappa Light Chain

Immunogen

Immunogen
Synthetic peptide corresponding to an internal region of human Wnt1 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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