Monoclonal Rat anti-Mouse CD90.2 (Mouse) Antibody (clone 30H12, AF647)
Catalog Number: LS-C810655
2 sizes / from $259.00
Antibody: Rat Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse
Format: Liquid
Monoclonal Mouse anti-Human VWF / Von Willebrand Factor Antibody (clone RFF-VIII R/2, IHC)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Frozen, Other, Radioimmunoassay |
| Reactivity: | Human |
| Format: | Liquid |
$519.00each
Catalog Number: LS-C188528-0.5
Catalog Number: LS-C188528
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- Description
- Von Willebrand Factor antibody LS-C188528 is an unconjugated mouse monoclonal antibody to human Von Willebrand Factor (VWF). Validated for ELISA, IHC, Purif and RIA.
- Application
- ELISA, IHC-F, Other, RIA
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Coagulation factor VIII VWF | F8VWF | VWD | VWF | Von Willebrand factor | factor VIII-related antigen | Factor VIII-R | Factor VIII-R Ag | VIII R AG
- Recommended Dilution: IHC-F
- 1:25 - 1:100
- UniProt Number
- P04275
- NCBI GenBank
- NM_000552 | NP_000543.2
- SwissProt
- P04275
Target
- Target
- Human VWF / Von Willebrand Factor
- Antigen Species
- Human
- Epitope
- Recognizes human von Willebrand factor (vWF), also known as Factor VIII related antigen, a blood glycoprotein involved in blood coagulation. It stabilizes circulating Factor VIII by binding to it and protecting it from cleavage and delivers it to sites of vascular injury. vWF also promotes the adhesion of platelets to sites of vascular damage by forming a molecular bridge between collagen on exposed endothelial cells and the GPIb binding sites of platelets circulating in the blood. vWF circulates in the blood as large multimers, with each monomer (250kD) containing a number of specific domains. Hereditary or acquired defects in vWF lead to von Willebrand disease (vWD), characterized by varying degrees of susceptibility to bleeding. Symptoms might include nosebleeds, bleeding gums, easy bruising, menorrhagia or gastrointestinal bleeding. Various forms of vWD exist with differing severities, determined by the type of defect. Clone RF-VIII R/2 has a high affinity for an epitope within the platelet GPIb-binding site that is responsible for biological activity. As such the antibody is a potent inhibitor of vWF activity. It can completely neutralize ristocetin-induced platelet aggregation, platelet binding to the subendothelium and ristocetin-induced binding of vWF to platelets. It also inhibits platelet adhesion to glass beads. The epitope recognized is present only on the intact multimeric form of vWF and is abolished by mild denaturation with SDS. The antibody does not recognize human Factor VIII. Clone RF-VIII R/2 may be used to detect vWF in immunoassays in combination with clone RFF-VIII R/1 as a capture reagent.
- Gene Name
- VWF
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- RFF-VIII R/2
- Isotype
- IgG1
Immunogen
- Immunogen
- Human Factor VIII complex partially purified from Factor VIII concentrate.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
- Recommended Storage Buffer
- PBS
