Overlay histogram showing A549 cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Monoclonal Mouse anti-Human SOD2 / Mn SOD Antibody (clone 1559CT572.3.83, IHC, WB)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$401.00each

Catalog Number: LS-C392600-200

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Mn SOD antibody LS-C392600 is an unconjugated mouse monoclonal antibody to Mn SOD (SOD2) from human. It is reactive with human and mouse. Validated for Flow, IHC, Peptide-ELISA and WB.
Application: ELISA, FC, IHC, IHC-P, WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: IPOB | MVCD6 | Indophenoloxidase B | SOD2 | Mn superoxide dismutase | MNSOD | Mangano-superoxide dismutase
Recommended Dilution: FC: 1:25
Recommended Dilution: IHC-P: 1:25
Recommended Dilution: WB: 1:2000
UniProt Number: P04179
NCBI GenBank: NM_000636 | NP_000627.2
SwissProt: P04179

Target

Target: Human SOD2 / Mn SOD
Antigen Species: Human
Epitope: This SODM antibody is generated from a mouse immunized with a recombinant protein of human SODM.
Gene Name: SOD2

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 1559CT572.3.83
Isotype: IgG1
Light Chain: Kappa Light Chain

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, 0.09% Sodium Azide
Volume: 200 Microliter
Preservative: Yes
Purification Method: Protein G Affinity

Storage & Handling

Storage Conditions: Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer: PBS