LSBio
Monoclonal Mouse anti-Human SERPINB9 / PI9 Antibody (clone 7D8, IHC, WB)

Monoclonal Mouse anti-Human SERPINB9 / PI9 Antibody (clone 7D8, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Human SERPINB9 / PI9 antibodies →
$413.00each
Catalog Number: LS-C44956-0.1

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Overview

Description
PI9 antibody LS-C44956 is an unconjugated mouse monoclonal antibody to human PI9 (SERPINB9). Validated for Flow, IHC and WB.
Application
FC, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CAP-3 | CAP3 | Cytoplasmic antiproteinase 3 | Peptidase inhibitor 9 | Serpin B9 | PI-9 | SERPINB9 | PI9
Recommended Dilution: FC
1:10 - 1:100
Recommended Dilution: IHC-P
1:50
UniProt Number
P50453
NCBI GenBank
NM_004155 | NP_004146.1
SwissProt
P50453

Target

Target
Human SERPINB9 / PI9
Antigen Species
Human
Epitope
Specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kD intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone Detects a band of approximately 42kD under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence.
Gene Name
SERPINB9

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
7D8
Isotype
IgG1

Immunogen

Immunogen
Recombinant PI-9 produced in P. pastoris.
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.05% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.