Anti-PMS2 Antibody - Western Blot. Western blot analysis is shown using Protein A Purified Mouse Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. Approximately 5,10 and 30 ug of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer. The blot was incubated with a 1:1000 dilution of the antibody at room temperature followed by washing. A 1:20000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemicals SuperSignal substrate. Comparison to a molecular weight marker (not shown) indicates a single band of ~96.0 kD corresponding to the expected molecular weight for human PMS2 protein. Other detection systems will yield similar results. Personal communication Morphotek Inc.