LSBio
Monoclonal Mouse anti-Human MAPK3 / ERK1 Antibody (phospho-Thr202/Tyr204, IF, WB)

Monoclonal Mouse anti-Human MAPK3 / ERK1 Antibody (phospho-Thr202/Tyr204, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Reactivity:Hamster, Human, Mouse, Rat
Format:Liquid
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$708.00each
Catalog Number: LS-C16353-200

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Overview

Description
ERK1 antibody LS-C16353 is an unconjugated mouse monoclonal antibody to ERK1 (MAPK3) (pThr202/Tyr204) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, ICC, IF, IP and WB.
Application
ELISA, FC, ICC, IF, IP, WB
Reactivity
Ham, Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | MAPK3 | PRKM3
Recommended Dilution: FC
1:1000
Recommended Dilution: IF/ICC
1:200|0.180555555555556
Recommended Dilution: IP
1:500
Recommended Dilution: WB
1:2000
UniProt Number
P27361
NCBI GenBank
CAA42744.1|X60188
SwissProt
P27361

Target

Target
Human MAPK3 / ERK1
Antigen Species
Human
Epitope
Detects endogenous levels of human p44 and p42 MAP kinase (Erk1 and Erk2) dually phosphorylated at threonine 202 and tyrosine 204. Does not cross-react with the corresponding phosphorylated residues of either SAPK/JNK or p38 MAP kinase. Species cross-reactivity: Hamster, mouse, rat and zebrafish.
Gene Name
MAPK3

Antibody

Host Species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Origin Species
Human

Immunogen

Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Volume
200 Microliter
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
10 mM HEPES|150 mM NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.