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Monoclonal Mouse anti-Human MAPK1 / ERK2 Antibody (phospho-Thr180/Tyr182, WB)

Monoclonal Mouse anti-Human MAPK1 / ERK2 Antibody (phospho-Thr180/Tyr182, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunoprecipitation, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human MAPK1 / ERK2 antibodies →
$732.00each
Catalog Number: LS-C16335-100

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Overview

Description
ERK2 antibody LS-C16335 is an unconjugated mouse monoclonal antibody to ERK2 (MAPK1) (pThr180/Tyr182) from human. It is reactive with human, mouse and rat. Validated for Flow, IP and WB.
Application
FC, IP, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
ERK | ERK-2 | ERK2 | MAP kinase 1 | MAPK 2 | MAPK2 | MAP kinase isoform p42 | MAPK 1 | MAPK1 | p41mapk | PRKM2 | MAP kinase 2 | p41 | p42-MAPK | PRKM1 | ERT1 | p38 | p40 | p42MAPK | Protein tyrosine kinase ERK2
Recommended Dilution: FC
1:1000
Recommended Dilution: IP
1:50
Recommended Dilution: WB
1:2000
UniProt Number
P28482
SwissProt
P28482

Target

Target
Human MAPK1 / ERK2
Antigen Species
Human
Epitope
Detects p38 MAP kinase only when activated by dual phosphorylation at Thr180 and Tyr182. Does not appreciably cross-react with the corresponding phosphorylated forms of either p44/42 MAPK (Erk1/2) or SAPK/JNK. It does not detect non-phosphorylated p38 MAP kinase. Species cross-reactivity: Human, mouse and rat.
Gene Name
MAPK1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Origin Species
Human

Immunogen

Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr180/Tyr182 of human p38 MAP kinase.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol
Volume
100 Microliter
Preservative
No
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
10 mM HEPES|150 mM NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.