Monoclonal Rat anti-Mouse CD90.2 (Mouse) Antibody (clone 30H12, AF647)
Catalog Number: LS-C810655
2 sizes / from $259.00
Antibody: Rat Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse
Format: Liquid
Anti-Human IL-6 Antibody - Flow Cytometry Human PBMCs were stimulated with 1ug/mL LPS and a transport inhibitor for 4-5 hours. Cells were then suspended in fixation buffer for 10-12 minutes and vortexed briefly. 1mL of permeabilization buffer was added. 0.5mg of Anti-Human IL-6 Antibody was added (0.125 ug/mL control antibody) and incubated in the dark for 30 minutes. 1:100 of strep/PE was added and incubated for 30 minutes. LPS-stimulated samples were compared to unstimulated cells stained with strep/PE.
Monoclonal Mouse anti-Human IL6 / Interleukin 6 Antibody (clone 33A12.G9, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745223-25
Catalog Number: LS-C745223
For bulk orders or custom formulations:
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- Description
- Interleukin 6 antibody LS-C745223 is an unconjugated mouse monoclonal antibody to human Interleukin 6 (IL6). Validated for ELISA, Flow and WB.
- Application
- ELISA, FC, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- B-cell stimulatory factor 2 | BSF-2 | BSF2 | CTL differentiation factor | Hybridoma growth factor | IFN-beta-2 | IL6 | Interleukin-6 | Interleukin BSF-2 | IFNB2 | B-cell differentiation factor | CDF | HSF | IL-6
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: FC
- 0.5 mg/ml
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P05231
- NCBI GenBank
- NM_000600, NP_000591.1
- SwissProt
- P05231
Target
- Target
- Human IL6 / Interleukin 6
- Antigen Species
- Hu
- Gene Name
- IL6
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 33A12.G9
- Isotype
- IgG1
- Light Chain
- κ
- Origin Species
- Hu
Immunogen
- Immunogen
- IL-6 monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development.
- Immunogen Type
- FL-RP
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Crude
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
