LSBio
Monoclonal Mouse anti-Human HMG1 / HMGB1 Antibody (clone J2E1, aa1-215, IHC, WB)

Cell lysates of Jurkat and HeLa (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HMGB1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Monoclonal Mouse anti-Human HMG1 / HMGB1 Antibody (clone J2E1, aa1-215, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human HMG1 / HMGB1 antibodies →
$310.00each
Catalog Number: LS-C93743-50

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Overview

Description
HMGB1 antibody LS-C93743 is an unconjugated mouse monoclonal antibody to human HMGB1 (HMG1) (aa1-215). Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Amphoterin | High mobility group protein B1 | High mobility group box 1 | High mobility group protein 1 | HMG1 | HMG3 | SBP-1 | High-mobility group box 1 | HMG-1 | HMGB1
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P09429
NCBI GenBank
NM_002128 | NP_002119.1
SwissProt
P09429

Target

Target
Human HMG1 / HMGB1
Antigen Species
Human
Gene Name
HMGB1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
J2E1
Isotype
IgG2b
Origin Species
Human

Immunogen

Immunogen
Recombinant human HMGB1 (1-215aa) purified from High Five (Trichoplusia ni) insect cells. NCBI Accession No.: NP_002119.
Immunogen Type
Recombinant Protein Fragment

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.1% Sodium Azide
Concentration
1 mg/mL
Volume
50 Microliter
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.