LSBio
Monoclonal Mouse anti-Human HLA-G Antibody (clone 87G, Low Endotoxin, IHC)

Analysis of cytolytical activity of human polyclonal NK cells on target melanoma cells. Blocking of HLA-G1 on transfectants with anti-human HLA-G (87G) restored specific lysis. Target cells: M8 cell line transfected with empty vector (column 1) and with HLA-G1 cDNA (columns 2-6). Blocking antibodies: Column 1-2: none Column 3: Isotype mouse IgG2a control Column 4: anti-human HLA-G (87G) purified Column 5: anti-human HLA-G (87G) F(ab')2 fragment Column 6: anti-human HLA-G (MEM-G/9)

Monoclonal Mouse anti-Human HLA-G Antibody (clone 87G, Low Endotoxin, IHC)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Functional Assay, Immunohistochemistry (general), Immunohistochemistry, Frozen
Reactivity:Human
Format:Liquid
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$354.00each
Catalog Number: LS-C204839-0.1

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Overview

Description
HLA-G antibody LS-C204839 is an unconjugated mouse monoclonal antibody to human HLA-G. Validated for ELISA, Flow, Func and IHC.
Application
ELISA, FC, Func, IHC, IHC-F
Reactivity
Hu
Nonreactive Species
Mouse, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
HLAG | HLA-6.0 | MHC class I antigen G | HLA G antigen
UniProt Number
P17693
SwissProt
P17693

Target

Target
Human HLA-G
Antigen Species
Human
Epitope
The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. The antibody 87G blocks interaction of HLA-G with inhibitory receptors.
Gene Name
HLA-G

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
87G
Isotype
IgG2a

Immunogen

Immunogen
HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Sterile PBS, pH 7.4, Azide free
Concentration
1 mg/mL
Preservative
No
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C. Do not freeze.
Recommended Storage Buffer
PBS

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