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Monoclonal Mouse anti-Human Histone H4 Antibody (clone 5E10-D8, WB)

Anti-H4-me1K20, clone 5E10-D8. HeLa histones were separated by 15% SDS-PAGE and blotted onto nitrocellulose membrane (Schleicher&Schull). The membrane was blocked in 3% NFDM in PBS-T for 1h at RT and incubated at 4°C with anti-histone H4-me1K20, clone 5E10-D8 antibody at the indicated dilutions in 0.5% NFDM/PBS-T. After incubation with anti-mouse Fc-HRP coupled secondary antibody (Jackson Immunoresearch) for 1h at RT. ECL was performed with the Western Lightning reagents (Perkin Elmer). A 3 min exposure is shown.

Monoclonal Mouse anti-Human Histone H4 Antibody (clone 5E10-D8, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Chromatin Immunoprecipitation, Dot Blot, Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
See all Human Histone H4 antibodies →
$492.00each
Catalog Number: LS-C827847-0.01

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Overview

Description
Histone H4 antibody LS-C827847 is an unconjugated mouse monoclonal antibody to human Histone H4. Validated for ChrIP, DB, ELISA and WB.
Application
ChIP, DB, ELISA, WB
Reactivity
Hu
Predicted Reactivity
Dros, Mml, Plnt, Xen
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies

Target

Target
Human Histone H4
Antigen Species
Hu
Epitope
Specific for K20 mono-methylated histone H4; no cross-reactivity with K20 tri-, di-, or unmethylated histone H4; no cross-reactivity with histone H3.

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
5E10-D8
Isotype
IgG1

Immunogen

Immunogen
Synthetic peptide of Human Histone H4
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, 0.1% Sodium Azide
Preservative
Yes
Purification Method
Prot G

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.