The recombianat protein (20ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human Hexokinase 1 Lane 2 : Recombinant human Hexokinase 2 Lane 3 : Recombinant human Hexokinase 3 Lane 4 : Recombinant human Hexokinase 4 The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: K562 cell lysate Lane 4.: Mouse brain tissue lysate