LSBio
Monoclonal Mouse anti-Human Heat Shock Protein 70 / HSPA1A Antibody (clone 1H11, IF, WB)

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100. Counterstain: Wheat germ agglutinin Texas red®membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, cell membrane. (A) DAPI nuclear stain. (B) Wheat germ agglutinin Texas red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.

Monoclonal Mouse anti-Human Heat Shock Protein 70 / HSPA1A Antibody (clone 1H11, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunofluorescence, Western Blot
Reactivity:Bovine, Human, Mouse, Pig, Rat
Format:Liquid
See all Human Heat Shock Protein 70 / HSPA1A antibodies →
$496.00each
Catalog Number: LS-C773132-100

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Overview

Description
HSPA1A antibody LS-C773132 is an unconjugated mouse monoclonal antibody to HSPA1A (Heat Shock Protein 70) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF and WB.
Application
FC, IF, WB
Reactivity
Bov, Hu, Ms, Por, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: FC
1:250
Recommended Dilution: IF/ICC
1:100
Recommended Dilution: WB
1:1000

Target

Target
Human Heat Shock Protein 70 / HSPA1A
Antigen Species
Hu
Epitope
Conformation-specific antibody for membrane-bound HSP70. Binds to cell surface HSP70 without permeabilization of cell membrane in tumor cell lines. Does not bind to intracellular HSP70 at low concentrations. Cross-reacts with HSC70. Detects a ~70 kDa band in Western Blot.

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
1H11
Isotype
IgG1

Immunogen

Immunogen
Native human Hsp70 protein.
Immunogen Type
Native

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Concentration
0.5 mg/mL
Preservative
Yes
Purification Method
Prot G

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.