LSBio
Monoclonal Mouse anti-Human HBB / Hemoglobin Beta Antibody (clone 14G2.G11.F11, N-Terminus, WB)

Western Blot of Mouse Anti-Hemoglobin A (beta chain) Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. Lane 6: HbS peptide conjugated to BSA. Load: 50ng per lane. Primary antibody: Anti-HbA antibody at 1µg/mL overnight at 4°C. Secondary antibody: Rabbit Anti-Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-073 for 30 min RT. Predicted/Observed: Reactivity seen in Lane 2 specific to HbA and cross reactivity to HbA-2 seen in Lane 3.

Monoclonal Mouse anti-Human HBB / Hemoglobin Beta Antibody (clone 14G2.G11.F11, N-Terminus, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
See all Human HBB / Hemoglobin Beta antibodies →
$311.00each
Catalog Number: LS-C744863-25

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Overview

Description
Hemoglobin Beta antibody LS-C744863 is an unconjugated mouse monoclonal antibody to human Hemoglobin Beta (HBB) (N-Terminus). Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CD113t-C | Hemoglobin beta | HBB | Hemoglobin beta chain | Hemoglobin subunit beta | Beta globin chain | Beta-globin | Hemoglobin, beta
Recommended Dilution: ELISA
1:20000
Recommended Dilution: WB
1 µg/ml
UniProt Number
P68871
SwissProt
P68871

Target

Target
Human HBB / Hemoglobin Beta
Antigen Species
Hu
Gene Name
HBB

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
14G2.G11.F11
Isotype
IgG2a
Light Chain
λ
Origin Species
Hu

Immunogen

Immunogen
Anti-Hemoglobin A (beta chain) Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-Terminus of Hb β-subunit conjugated to KLH.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Prot A

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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