Antibody staining CDK4 in human breast carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Monoclonal Mouse anti-Human CDK4 Antibody (clone 1529CT850.162.73, IHC, WB)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$401.00each

Catalog Number: LS-C392591-200

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: CDK4 antibody LS-C392591 is an unconjugated mouse monoclonal antibody to CDK4 from human. It is reactive with human and mouse. Validated for IHC, Peptide-ELISA and WB.
Application: ELISA, IHC, IHC-P, WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: CDK4 | Cell division protein kinase 4 | Crk3 | Cyclin-dependent kinase 4 | CMM3 | p34/cdk4 | PSK-J3
Recommended Dilution: IHC-P: 1:25
Recommended Dilution: WB: 1:2000
UniProt Number: P11802
NCBI GenBank: NM_000075 | NP_000066.1
SwissProt: P11802

Target

Target: Human CDK4
Antigen Species: Human
Epitope: This CDK4 antibody is generated from a mouse immunized with a recombinant protein.
Gene Name: CDK4

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 1529CT850.162.73
Isotype: IgG2a
Light Chain: Kappa Light Chain

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, 0.09% Sodium Azide
Concentration: 0.3 mg/mL
Volume: 200 Microliter
Preservative: Yes
Purification Method: Protein G Affinity

Storage & Handling

Storage Conditions: Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer: PBS