LSBio
Monoclonal Mouse anti-Human CD3E Antibody (clone APA1/1, PE, IHC, WB)

Intracellular staining of JURKAT cells with anti-CD3 epsilon activation epitope (APA1/1) PE.

Monoclonal Mouse anti-Human CD3E Antibody (clone APA1/1, PE, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Frozen, Immunoprecipitation, Western Blot
Reactivity:Human, Mouse
Format:Liquid
See all Human CD3E antibodies →
$373.00each
Catalog Number: LS-C112557-0.1

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Overview

Description
CD3E antibody LS-C112557 is a PE-conjugated mouse monoclonal antibody to CD3E from human. It is reactive with human and mouse. Validated for Flow, ICC, IHC, IP and WB.
Application
FC, ICC, IHC-F, IP, WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CD3e antigen | CD3E | CD3-epsilon | T-cell receptor epsilon chain | TCRE | T-cell receptors epsilon chain | T3E | CD3 Epsilon | CD3
NCBI GenBank
NM_000733 | NP_000724.1

Target

Target
Human CD3E
Antigen Species
Human
Epitope
The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
APA1/1
Isotype
IgG1

Immunogen

Immunogen
Purified human CD3 proteins isolated from thymus.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Phycoerythrin
Format
Liquid
Formulation
PBS, 15mM sodium azide
Concentration
0.1 mg/mL
Preservative
Yes
Purification Method
Size Exclusion Chromatography

Storage & Handling

Storage Conditions
Store at 2°C to 8°C. Do not freeze. Avoid prolonged exposure to light.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.