LSBio
Monoclonal Mouse anti-Human CCNE1 / Cyclin E1 Antibody (clone 03, Preservative Free, IF)

Immunofluorescence staining of CCNE1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-human CCNE1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleolus.

Monoclonal Mouse anti-Human CCNE1 / Cyclin E1 Antibody (clone 03, Preservative Free, IF)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunocytochemistry, Immunofluorescence
Reactivity:Human
Format:Liquid
See all Human CCNE1 / Cyclin E1 antibodies →
$309.00each
Catalog Number: LS-C819859-100

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Overview

Description
Cyclin E1 antibody LS-C819859 is an unconjugated mouse monoclonal antibody to human Cyclin E1 (CCNE1). Validated for Flow, ICC and IF.
Application
FC, ICC, IF
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CCNE1 | Cyclin E1 | CCNE | Cyclin Es | Cyclin Et | G1/S-specific cyclin-E1 | G1/s-specific cyclin e | Cyclin E
Recommended Dilution: FC
1:25 - 1:100
Recommended Dilution: IF/ICC
1:20 - 1:100
UniProt Number
P24864
NCBI GenBank
NM_001238 | NP_001229.1
SwissProt
P24864

Target

Target
Human CCNE1 / Cyclin E1
Antigen Species
Human
Epitope
Human Cyclin E
Gene Name
CCNE1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
03
Isotype
IgG2b
Origin Species
Human

Immunogen

Immunogen
Recombinant Human Cyclin E/CCNE1 Protein
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.2 µm filtered solution in PBS
Volume
100 Microliter
Preservative
No
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

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