LSBio
Monoclonal Mouse anti-All species Citrulline Antibody (clone 2D3.1, IF, WB)

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3.1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Monoclonal Mouse anti-All species Citrulline Antibody (clone 2D3.1, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity:Pan-species / Cross-reactive
Format:Liquid
See all All species Citrulline antibodies →
$507.00each
Catalog Number: LS-C775577-100

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Overview

Description
Citrulline antibody LS-C775577 is an unconjugated mouse monoclonal antibody to all species Citrulline. Validated for ELISA, IF and WB.
Application
ELISA, IF, WB
Reactivity
Pan
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:1000
Recommended Dilution: IF/ICC
1:50
Recommended Dilution: WB
1:1000

Target

Target
All species Citrulline
Antigen Species
Pan
Epitope
Specific for citrulline residues on modified proteins. Tested using citrulline conjugated Bovine Serum Albumin (BSA).

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
2D3.1
Isotype
IgG1

Immunogen

Immunogen
Synthetic L-Citrulline conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Synth

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Prot G

Storage & Handling

Storage Conditions
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.