Anti-Human MIF Polyclonal Antibody - Western Blot. Western blot of IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 ng of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kD. A 4-20% gradient gel was used to separate the proteins by SDS-PAGE. The protein was transferred to nitro-cellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Chicken Rabbit IgG [H&L] (code for 1 h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.