LSBio
MHC Class II I-A/I-E Antibody (clone M5/114, Azide-free)

Surface staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.

MHC Class II I-A/I-E Antibody (clone M5/114, Azide-free)

Primary AntibodyLSBio Guarantee
Antibody:Rat Monoclonal
Applications:Flow Cytometry, Functional Assay, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Mouse
Format:Liquid
See all Mouse MHC Class II I-A/I-E antibodies →
$301.00each
Catalog Number: LS-C204829-0.1

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Overview

Description
I-E antibody LS-C204829 is an unconjugated rat monoclonal antibody to mouse I-E (MHC Class II I-A). Validated for Flow, Func, IHC, IP and WB.
Application
FC, Func, IHC, IHC-F, IHC-P, IP, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies

Target

Target
Mouse MHC Class II I-A/I-E
Antigen Species
Mouse
Epitope
The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

Antibody

Host Species
Rat
Clonality
Monoclonal
Clone
M5/114
Isotype
IgG2b

Immunogen

Immunogen
Activated C57BL/6 mouse spleen cells.
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Sterile PBS, pH 7.4, Azide free
Concentration
1 mg/mL
Preservative
No
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C. Do not freeze.
Recommended Storage Buffer
PBS

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