Flow Cytometry analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
MGO / Methylglyoxal Antibody (clone 9F11)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Western Blot |
| Reactivity: | Pan-species / Cross-reactive |
| Format: | Liquid |
$507.00each
Catalog Number: LS-C775648-100
Catalog Number: LS-C775648
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Methylglyoxal antibody LS-C775648 is an unconjugated mouse monoclonal antibody to all species Methylglyoxal (MGO). Validated for ELISA, Flow, IF and WB.
- Application
- ELISA, FC, IF, WB
- Reactivity
- Pan
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: ELISA
- 1:1000
- Recommended Dilution: FC
- 1:50
- Recommended Dilution: IF/ICC
- 1:50
- Recommended Dilution: WB
- 1:1000
Target
- Target
- All species MGO / Methylglyoxal
- Antigen Species
- Pan
- Epitope
- Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 9F11
- Isotype
- IgG1
Immunogen
- Immunogen
- Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).
- Immunogen Type
- Synth
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS