Western Blot analysis of Methylglyoxal-BSA Conjugate showing detection of 67 kDa Methylglyoxal protein using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Lane 1: Molecular Weight Ladder (MW). Lane 2: Methylglyoxal-BSA. Lane 3: BSA. Load: 0.5 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
MGO / Methylglyoxal Antibody (clone 9E7)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Pan-species / Cross-reactive |
| Format: | Liquid |
$507.00each
Catalog Number: LS-C775666-100
Catalog Number: LS-C775666
For bulk orders or custom formulations:
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- Description
- Methylglyoxal antibody LS-C775666 is an unconjugated mouse monoclonal antibody to all species Methylglyoxal (MGO). Validated for ELISA, Flow, IF, IHC and WB.
- Application
- ELISA, FC, IF, IHC, WB
- Reactivity
- Pan
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: ELISA
- 1:1000
- Recommended Dilution: FC
- 1:50
- Recommended Dilution: IF/ICC
- 1:50
- Recommended Dilution: IHC
- 1:25
- Recommended Dilution: WB
- 1:1000
Target
- Target
- All species MGO / Methylglyoxal
- Antigen Species
- Pan
- Epitope
- Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 9E7
- Isotype
- IgG2a
Immunogen
- Immunogen
- Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).
- Immunogen Type
- Synth
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS