Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using MEK1/2(Phospho-Ser218/222) antibody.
MEK1 + MEK2 Antibody (phospho-Ser217)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C827225-100
Catalog Number: LS-C827225
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- Description
- MEK1 + MEK2 antibody LS-C827225 is an unconjugated rabbit polyclonal antibody to MEK1 + MEK2 (pSer217) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: ELISA
- 1:4000
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
Target
- Target
- Human MEK1 + MEK2
- Antigen Species
- Human
- Epitope
- MEK1/2(Phospho-Ser218/222) antibody detects endogenous levels of MEK1 only when phosphorylated at serine Ser218/222. (Human: Ser218/Ser222; Mouse: Ser218/Ser222; Rat: Ser218/Ser222)
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of serine Ser218/222(I-D-SP-M-A).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl