Western Blot of Anti-MEK1 pS222 Antibody. Lane 1: MEK-1 recombinant protein. Lane 2: MEK-2 recombinant protein. Load: 50ng per lane. Primary Antibody: Anti-MEK1 pS222 supernatant clone neat over night at 4°C. Secondary Antibody: Anti-mouse HRP at 1:40,000 dilution.
MEK1 + MEK2 Antibody (MEK1 phospho-Ser222 and MEK2 pSer226, clone 17C9.E1.H2.D7.H7.D5)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C744873-100
Catalog Number: LS-C744873
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- Description
- MEK1 + MEK2 antibody LS-C744873 is an unconjugated mouse monoclonal antibody to MEK1 + MEK2 (MEK1 pSer222 and MEK2 pSer226) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: ELISA
- 1:40000
- Recommended Dilution: WB
- 1 µg/ml
Target
- Target
- Human MEK1 + MEK2
- Antigen Species
- Human
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 17C9.E1.H2.D7.H7.D5
- Isotype
- IgG1
- Light Chain
- Lambda Light Chain
Immunogen
- Immunogen
- Anti-MEK1 pS222/MEK2 pS226 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues surrounding the S222-226 phosphorylation site conjugated to KLH.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl