Western blot analysis of extracts of various cell lines, using MED22 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

MED22 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$281.00each

Catalog Number: LS-C748849-20

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: MED22 antibody LS-C748849 is an unconjugated rabbit polyclonal antibody to MED22 from human. It is reactive with human and mouse. Validated for WB.
Application: WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Mediator complex subunit 22 | Surf-5 | SURF5 | Surfeit 5 | Surfeit locus protein 5 | MED22
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q15528
SwissProt: Q15528

Target

Target: Human MED22
Antigen Species: Human
Gene Name: MED22

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human MED22 (NP_852468.1). MAQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISIDLYELEEEYYSSRYK
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)