Western blot analysis of extracts of various cell lines, using MED21 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.

MED21 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$281.00each

Catalog Number: LS-C747340-20

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Overview

Description: MED21 antibody LS-C747340 is an unconjugated rabbit polyclonal antibody to MED21 from human. It is reactive with human and mouse. Validated for WB.
Application: WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: HSrb7 | RNAPII complex component SRB7 | SRB7 | MED21 | SURB7 | Mediator complex subunit 21
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q13503
NCBI GenBank: NM_004264 | NP_004255.2
SwissProt: Q13503

Target

Target: Human MED21
Antigen Species: Human
Gene Name: MED21

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human MED21 (NP_004255.2). MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQLKTRSGTHSQSLPDS
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)