Western Blot of Sheep anti-Malate Dehydrogenase antibody. Lane 1: Malate Dehydrogenase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Malate Dehydrogenase antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase sheep secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 36 kDa, 36 kDa for Malate Dehydrogenase. Other band(s): None.
ME1 / Malate Dehydrogenase Antibody (Liquid)
Primary AntibodyLSBio Guarantee
| Antibody: | Sheep Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Pig |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745165-25
Catalog Number: LS-C745165
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- Description
- Malate Dehydrogenase antibody LS-C745165 is an unconjugated sheep polyclonal antibody to pig Malate Dehydrogenase (ME1). Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Por
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HUMNDME | Malate dehydrogenase | Malic enzyme 1 | Malate oxidoreductase | Malic enzyme 1, soluble | ME1 | MES | NADP-ME | Pyruvic-malic carboxylase | NADP-dependent malic enzyme | Malic enzyme, cytoplasmic
- Recommended Dilution: ELISA
- 1:5000 - 1:20000
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:5000
- UniProt Number
- P48163
- NCBI GenBank
- NM_002395 | NP_002386.1
- SwissProt
- P48163
Target
- Target
- Pig ME1 / Malate Dehydrogenase
- Antigen Species
- Pig
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
- Gene Name
- ME1
Antibody
- Host Species
- Sheep
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Malate Dehydrogenase [Pig Heart].
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl