LSBio
MDM2 Antibody (aa151-200)

Immunofluorescence analysis of NIH/3T3 cells, using MDM2 Antibody. The picture on the right is blocked with the synthesized peptide.

MDM2 Antibody (aa151-200)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Human MDM2 antibodies →
$341.00each
Catalog Number: LS-C199239-50

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Overview

Description
MDM2 antibody LS-C199239 is an unconjugated rabbit polyclonal antibody to human MDM2 (aa151-200). Validated for IF, IHC and Peptide-ELISA. Cited in 3 publications.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ACTFS | Double minute 2 protein | Hdm2 | MDM2 variant FB30 | MDM2 | HDMX | Oncoprotein Mdm2 | p53-binding protein Mdm2 | MDM2 variant FB28
Recommended Dilution: IF/ICC
1:100 - 1:500
UniProt Number
Q00987
SwissProt
Q00987

Target

Target
Human MDM2
Antigen Species
Human
Epitope
MDM2 (Ab-186/188) Antibody detects endogenous levels of total MDM2 protein.
Gene Name
MDM2

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Synthetic peptide from human MDM2. (AA Range: 151-200)
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
50 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS|150 mM NaCl

Stabilization of HIF-1a and HIF-2a, up-regulation of MYCC and accumulation of stabilized p53 constitute hallmarks of CNS-PNET animal model. Malchenko S, Sredni ST, Bi Y, Margaryan NV, Boyineni J, Mohanam I, Tomita T, Davuluri RV, Soares MB. PloS one. 2017;12:e0173106.

PubMed: 28249000 · PMC: PMC5332108


Characterization of brain tumor initiating cells isolated from an animal model of CNS primitive neuroectodermal tumors. Malchenko S, Sredni ST, Boyineni J, Bi Y, Margaryan NV, Guda MR, Kostenko Y, Tomita T, Davuluri RV, Velpula K, Hendrix MJC, Soares MB. Oncotarget. 2018 February;9:13733-13747.

PubMed: 29568390 · PMC: PMC5862611


Targeting the TP53/MDM2 axis enhances radiation sensitivity in atypical teratoid rhabdoid tumors. Irina Alimova, Dong Wang, Etienne Danis, Angela Pierce, Andrew Donson, Natalie Serkova, Krishna Madhavan, Senthilnath Lakshmanachetty, Ilango Balakrishnan, Nicholas K Foreman, Siddhartha Mitra, Sujatha Venkataraman, Rajeev Vibhakar. International journal of oncology. 2022 March;60:32.

PubMed: 35179215 · PMC: PMC8878716

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