Blot of crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector in lane labeled +. The - lane is a blot of an equal amount of protein extract from untransfected HEK293 cells. mCherry antibody binds a major band running at ~28kDa corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected HEK293 cells.