Immunofluorescence staining of U251 cells diluted at 1:37,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
MCAM / CD146 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Recombinant Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$323.00each
Catalog Number: LS-C675304-50
Catalog Number: LS-C675304
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- CD146 antibody LS-C675304 is an unconjugated rabbit recombinant monoclonal antibody to human CD146 (MCAM). Validated for ELISA, Flow, IF, IHC and WB.
- Application
- ELISA, FC, IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Recombinant Antibodies
- Alternative Names
- CD146 | CD146 antigen | Gicerin | Melanoma adhesion molecule | MCAM | MUC18 | p1h12
- UniProt Number
- P43121
- NCBI GenBank
- NM_006500 | NP_006491.2
- SwissProt
- P43121
Target
- Target
- Human MCAM / CD146
- Antigen Species
- Human
- Gene Name
- MCAM
Antibody
- Host Species
- Rabbit
- Clonality
- Recombinant Monoclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A synthesized peptide
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at -20°C. Long term: store at -80°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS|150 mM NaCl