Detection of Human and Mouse CD146 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CD146 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
MCAM / CD146 Antibody (aa600-650)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C290979-10
Catalog Number: LS-C290979
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- Description
- CD146 antibody LS-C290979 is an unconjugated rabbit polyclonal antibody to CD146 (MCAM) (aa600-650) from human. It is reactive with human and mouse. Validated for IP and WB.
- Application
- IP, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD146 | CD146 antigen | Gicerin | Melanoma adhesion molecule | MCAM | MUC18 | p1h12
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- P43121
- NCBI GenBank
- NM_006500 | NP_006491.2
- SwissProt
- P43121
Target
- Target
- Human MCAM / CD146
- Antigen Species
- Human
- Epitope
- Region between residue 600 to 650 of human Melanoma Adhesion Molecule using the numbering given in entry NP_006491.2 (GeneID 4162).
- Gene Name
- MCAM
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 4162 corresponding to amino acids between 600 and 650 of UniprotID: P43121
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate