Rb Anti-Maltose Binding Protein Antibody - Western Blot. Western Blot showing detection of Maltose Binding Protein (MBP) (0.05 ug) in Lane 2. MW markers indicated in Lane 1. Protein was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 1% BSA-TTBS (MB-013, diluted to 1X) 30 min at 20? Anti-MBP (RABBIT) antibody (p/n Anti-MALTOSE BINDING PROTEIN (MBP) EPITOPE TAG (RABBIT) Antibody) was used at 1:1000 overnight at 4?. Anti-Rabbit IgG (GOAT) IRDye800 conjugated antibody (p/n secondary antibody was used at 1:20000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070) for 30 min at 20? and imaged on the LiCor Odyssey imaging system. A band is present at the correct molecular weight, ~42 kD, the other bands present are recombinant MBP breakdown.