LSBio
MARCKS Antibody (phospho-Ser152/Ser156)

Western blot of rat brain lysate showing specific immunolabeling of the ~87k MARCKS protein phosphorylated at Serr152156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser152156 MARCKS. The immunolabeling is completely eliminated by treatment with l-Ptase.

MARCKS Antibody (phospho-Ser152/Ser156)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Western Blot
Reactivity:Bovine, Chicken, Human, Mouse, Rat, Xenopus
Format:Liquid
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$479.00each
Catalog Number: LS-C213-100

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Overview

Description
MARCKS antibody LS-C213 is an unconjugated rabbit polyclonal antibody to MARCKS (pSer152/Ser156) from rat. It is reactive with human, mouse, rat and other species. Validated for WB.
Application
WB
Reactivity
Bov, Ck, Hu, Ms, Rt, Xen
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
80K-L | 80K-L protein | MACS | MARCKS | MRACKS | Phosphomyristin | PKCSL | PRKCSL | p-Ser152 | 156 MARCKS
Recommended Dilution: WB
1:1000
UniProt Number
P29966
SwissProt
P29966

Target

Target
Rat MARCKS
Antigen Species
Rat
Epitope
Specific for the ~87k MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by X-phosphatase
Gene Name
MARCKS

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
A phosphopeptide corresponding to amino acid residues surrounding the phosphoSer152156 of MARCKS.
Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/mL BSA, 50% Glycerol
Volume
100 Microliter
Preservative
No
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: -20°C; Long term: -20°C.
Recommended Storage Buffer
10 mM HEPES|150 mM NaCl

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