1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
MAPKAPK5 / PRAK Antibody (phospho-Thr182)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$288.00each
Catalog Number: LS-C800215-100
Catalog Number: LS-C800215
For bulk orders or custom formulations:
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- Description
- PRAK antibody LS-C800215 is an unconjugated rabbit polyclonal antibody to PRAK (MAPKAPK5) (pThr182) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- MAPKAP kinase 5 | MAPKAP-K5 | MAPKAPK5 | MK5 | p38-regulated/activated kinase | MAPKAPK-5 | PRAK | MK-5 | p38-regulated protein kinase
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q8IW41
- NCBI GenBank
- NM_003668 | NP_003659.2
- SwissProt
- Q8IW41
Target
- Target
- Human MAPKAPK5 / PRAK
- Antigen Species
- Human
- Epitope
- Phospho-MAPKAPK5(Thr182) Antibody detects endogenous levels of MAPKAPK5 only when phosphorylated at Threonine 182.
- Gene Name
- MAPKAPK5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthesized peptide derived from human MAPKAPK5 around the phosphorylation site of Threonine 182.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS