Immunofluorescence staining of MAPK14 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-human MAPK14 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Nucleus.
MAPK14 / p38 Antibody (clone 07, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C820125-100
Catalog Number: LS-C820125
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- Description
- P38 antibody LS-C820125 is an unconjugated mouse monoclonal antibody to human p38 (MAPK14). Validated for ELISA, ICC, IF, IHC and WB.
- Application
- ELISA, ICC, IF, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CSAID-binding protein | CSBP2 | CSPB1 | CSBP1 | Csaids binding protein | CSBP | EXIP | MAP kinase 14 | MAP kinase p38 alpha | MAX-interacting protein 2 | MAPK 14 | MAPK14 | p38 alpha | PRKM15 | PRKM14 | RK | SAPK2A | MAP kinase MXI2 | Mxi2 | p38ALPHA | p38 | p38 MAP kinase | p38alpha Exip
- Recommended Dilution: ELISA
- 1:1000 - 1:2000
- Recommended Dilution: IF/ICC
- 1:20 - 1:100
- Recommended Dilution: IHC-P
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q16539
- NCBI GenBank
- NM_001315 | NP_001306.1
- SwissProt
- Q16539
Target
- Target
- Human MAPK14 / p38
- Antigen Species
- Human
- Epitope
- Human p38
- Gene Name
- MAPK14
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 07
- Isotype
- IgG2a
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human p38 alpha/MAPK14 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS