Western blot analysis of extracts of various cell lines, using MAPK12 antibody at 1:10000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
MAPK12 / ERK6 / SAPK3 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C748111-20
Catalog Number: LS-C748111
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- Description
- SAPK3 antibody LS-C748111 is an unconjugated rabbit polyclonal antibody to SAPK3 (MAPK12 / ERK6) from human. It is reactive with human and mouse. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ERK5 | ERK6 | ERK-6 | MAP kinase 12 | MAPK 12 | MAPK12 | MAP kinase p38 gamma | SAPK3 | p38-gamma | p38GAMMA | SAPK-3 | ERK3 | PRKM12
- Recommended Dilution: WB
- 1:2000 - 1:5000
- UniProt Number
- P53778
- NCBI GenBank
- NM_002969 | NP_002960.2
- SwissProt
- P53778
Target
- Target
- Human MAPK12 / ERK6 / SAPK3
- Antigen Species
- Human
- Gene Name
- MAPK12
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 248-367 of human MAPK12 (NP_002960.2). EFVQRLQSDEAKNYMKGLPELEKKDFASILTNASPLAVNLLEKMLVLDAEQRVTAGEALAHPYFESLHDTEDEPQVQKYDDSFDDVDRTLDEWKRVTYKEVLSFKPPRQLGARVSKETPL
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS