Anti-p42 MAP Kinase (ERK2) Antibody - Western Blot. Western blot of Affinity Purified anti-p42 MAP Kinase (ERK2) antibody shows detection of ERK2 in several whole cell lysates: HeLa (lane 1), A431 (lane 2), and NIH3T3 (lane 3). Detection occurs using a 1:1000 dilution of the primary antibody followed by 1:4000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure was approximately 1. Other detection systems will yield similar results.
MAPK1 / ERK2 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C18799-100
Catalog Number: LS-C18799
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- Description
- ERK2 antibody LS-C18799 is an unconjugated rabbit polyclonal antibody to human ERK2 (MAPK1). Validated for ELISA, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ERK | ERK-2 | ERK2 | MAP kinase 1 | MAPK 2 | MAPK2 | MAP kinase isoform p42 | MAPK 1 | MAPK1 | p41mapk | PRKM2 | MAP kinase 2 | p41 | p42-MAPK | PRKM1 | ERT1 | p38 | p40 | p42MAPK | Protein tyrosine kinase ERK2
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: WB
- 1:1000 - 1:,2000
- UniProt Number
- P28482
- NCBI GenBank
- NM_002745 | NP_002736.3
- SwissProt
- P28482
Target
- Target
- Human MAPK1 / ERK2
- Antigen Species
- Human
- Epitope
- This antiserum is directed against human p42 MAP Kinase (ERK2) protein and is useful in determining its presence by immunoblotting. No reactivity is observed against p44 MAP Kinase (ERK1). Cross reactivity is expected with p44 MAP Kinase (ERK1) proteins from human and mouse sources. Reactivity to rat tissues is also anticipated due to high sequence homology. Reactivity against homologues from other sources is not known.
- Gene Name
- MAPK1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Anti-Erk2 antibody was prepared by repeated immunizations with an Erk2 containing fusion protein. The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein. The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 85 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl