Western Blot of Mouse anti-ERK2 Antibody. Lane 1: Opal Pre-Stained Molecular Weight Ladder. Lane 2: ERK1 50ng. Lane 3: ERK2 50ng. Primary Antibody: Anti-ERK2 (internal) Monoclonal Antibody at 1µg/mL. Secondary Antibody: Rabbit anti-Mouse IgG HRP at 1:40,000. Blocking Buffer: BlockOut MB-073 for 30 min at RT. Expect: ~41kDa seen in lane 2 only.
MAPK1 / ERK2 Antibody (Internal, clone 3H9.A1.A8)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C744858-100
Catalog Number: LS-C744858
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- Description
- ERK2 antibody LS-C744858 is an unconjugated mouse monoclonal antibody to ERK2 (MAPK1) (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- ERK | ERK-2 | ERK2 | MAP kinase 1 | MAPK 2 | MAPK2 | MAP kinase isoform p42 | MAPK 1 | MAPK1 | p41mapk | PRKM2 | MAP kinase 2 | p41 | p42-MAPK | PRKM1 | ERT1 | p38 | p40 | p42MAPK | Protein tyrosine kinase ERK2
- UniProt Number
- P28482
- NCBI GenBank
- NM_002745 | NP_002736.3
- SwissProt
- P28482
Recommended Dilution
| Application | Dilution |
|---|---|
| ELISA | 1:40000 |
| WB | 1 µg/ml |
Target
- Target
- Human MAPK1 / ERK2
- Antigen Species
- Human
- Gene Name
- MAPK1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 3H9.A1.A8
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-ERK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl